Imaging Real-Time Gene Expression in Living Yeast
نویسندگان
چکیده
منابع مشابه
Imaging real-time gene expression in living yeast.
INTRODUCTIONThis protocol describes the application of the MS2 system to the yeast Saccharomyces cerevisiae. ASH1 mRNA tagged with six MS2 repeats (6MBSs) is used to follow the localization of the ASH1 mRNA particles to the bud tip of a haploid yeast cell. W303 yeast cells transformed with pG14-MS2-GFP and pGAL-lacZ-MS2-ASH1 are grown on select medium lacking tryptophan and leucine. RNA express...
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INTRODUCTIONThis protocol describes a method for establishing a green fluorescent protein (GFP) calibration curve using dilutions of recombinant GFP and blue fluorescent beads. The total fluorescence intensity (TFI) per mRNA molecule is first calculated by imaging serial dilutions of purified enhanced GFP (eGFP) to determine the TFI within a specific volume. A calibration curve of fluorescence ...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2007
ISSN: 1940-3402,1559-6095,1559-6095
DOI: 10.1101/pdb.prot4870